BTY6B15. RECOMBINANT DNA TECHNOLOGY AND BIOINFORMATICS
This course offered to the VIth Semester BSc Biotechnology students of University of Calicut.
Course Instructor: Dr. Mashhoor K.
Objectives of the course
To provide the idea on Recombinant DNA technology, its importance and application of recombinant DNA technology
Content:
1. Introduction to gene cloning, enzymes and basic tools involved in gene cloning. (5 hrs)
2. DNA sequencing methods, hybridization techniques (Northern, southern, western
blotting), In Situ hybridiztion, PCR (variation RtPCR), DNA finger printing- RFLP,
RAPD, AFLP and STR analysis. Isolation and purification of total cell DNA
(10 hrs)
3. Cloning vectors in prokaryotes and eukaryotes (pBr 322, puc 18, M13, cosmids, Phagemids, phasmids, yeast vectors, Animal viral vectors - SV40, Plant viral vectors - CaMV, Agrobacterium – Tiplasmid. (10 hrs)
4. Introduction of recombinant DNA into living cells an overview. Selection and screening
of recombinant clones.
(10 hrs)
5. Application of r-DNA technology - production of recombinant proteins, vaccines,
Transgenic plants. (Insect resistance, disease resistance), Transgenic animals - molecular
pharming.
(10 hrs)
6. Introduction to bioinformatics, pattern recognition and prediction, biological databases,
primary and secondary sequence databases, composite protein sequence databases, pair
wise alignment technique; database searching NCBI, EMB, FASTA, BLAST BITS etc.
algorithms and programmes, comparison of two sequences, global and local alignment –
multiple sequence alignment
(9 hrs)
