Biotechnology

BTY6B15. RECOMBINANT DNA TECHNOLOGY AND BIOINFORMATICS

This course offered to the VIth Semester BSc Biotechnology students of University of Calicut.

Course Instructor: Dr. Mashhoor K.

Objectives of the course

To provide the idea on Recombinant DNA technology, its importance and application of recombinant DNA technology

Content:

1. Introduction to gene cloning, enzymes and basic tools involved in gene cloning. (5 hrs)

 2. DNA sequencing methods, hybridization techniques (Northern, southern, western blotting), In Situ hybridiztion, PCR (variation RtPCR), DNA finger printing- RFLP, RAPD, AFLP and STR analysis. Isolation and purification of total cell DNA (10 hrs)

3. Cloning vectors in prokaryotes and eukaryotes (pBr 322, puc 18, M13, cosmids, Phagemids, phasmids, yeast vectors, Animal viral vectors - SV40, Plant viral vectors - CaMV, Agrobacterium – Tiplasmid. (10 hrs)

 4. Introduction of recombinant DNA into living cells an overview. Selection and screening of recombinant clones. (10 hrs) 5. Application of r-DNA technology - production of recombinant proteins, vaccines, Transgenic plants. (Insect resistance, disease resistance), Transgenic animals - molecular pharming. (10 hrs) 6. Introduction to bioinformatics, pattern recognition and prediction, biological databases, primary and secondary sequence databases, composite protein sequence databases, pair wise alignment technique; database searching NCBI, EMB, FASTA, BLAST BITS etc. algorithms and programmes, comparison of two sequences, global and local alignment – multiple sequence alignment (9 hrs)

             branch of science dealing with the study of all the life processes such as control and coordination within a living organism is called Biochemistry.

This term was introduced to us by Carl Neuberg, the father of biochemistry in the year 1930. This field combines biology as well as chemistry to study the chemical structure of a living organism. The biochemists get into the investigation of the chemical reactions and combinations which are involved in various processes like reproduction, heredity, metabolism, and growth, thus performing research in different kind of laboratories.

Introduction to Biochemistry includes wide areas of molecular biology as well as cell biology. It is relevant to molecules that make up the structure of organs and cells which is the molecular anatomy. It describes carbon compound and the reactions they undergo in living organisms. It also describes molecular physiology, which is the functions of molecules in carrying out the requirements of the cells and organs.

It mainly deals with the study of the structure and functions of the biomolecules such as the carbohydrates, proteins, acids, lipids. Hence, it is also called to as Molecular biology.